Figure 1. Sensitive detection using RealHelix™ Premier qPCR Kit [Green, Low ROX]. 10-fold serial-diluted genomic DNA as the standard sample were analyzed using the RealHelix™ Premier qPCR Kit for human glucose-6-phosphate dehydrogenase gene-specific primer set. The accuracy of real time PCR using the RealHelix™ Premier qPCR Kit is demonstrated by R² value=0.99887. NTC: Negative control. 

Figure 2. Amplification of target DNA with various G+C contents using RealHelix™ Premier qPCR Kit [Green, Low ROX]. It was evaluated by real-time PCR using 10-fold serial diluted human genomic DNA (red: 50 ng/rxn, green: 5 ng/rxn, blue: 0.5 ng/rxn) with each instruments. NTC: Negative control. 

Figure 3. Comparison of Real-time PCR using RealHelix™ Premier qPCR Kit [Green, Low ROX] with other company"s related products. 10-fold serial dilutions of the human genomic DNA (red: 50 ng/rxn, green: 5 ng/rxn, blue: 0.5 ng/rxn) from whole blood were analyzed using human HPRT-1 gene-specific primer set. Reactions were run on a real-time PCR instrument, CFX-96 (Bio-Rad, USA). NTC: Negative control.