Figure 1. Sensitive detection using Premier 2X qPCR Mix [Green] (= RealHelix^TM Premier qPCR Mix [Green]). 10-fold serial-diluted genomic DNA as the standard sample were analyzed using the Premier 2X qPCR Mix [Green] for human glucose-6-phosphate dehydrogenase gene-specific primer set. The accuracy of real time PCR using the Premier 2X qPCR Mix [Green] is demonstrated by R² value=0.99887. NTC: Negative control. 

Figure 2. Comparison of Real-time PCR using Premier 2X qPCR Mix [Green] (= RealHelix™ Premier qPCR Mix [Green]) iTaq Universal SYBR green supermix (company B) and TOPreal qPCR 2x premix (company E). 10-fold serial dilutions of the human genomic DNA (red: 50 ng/rxn, green: 5 ng/rxn, blue: 0.5 ng/rxn) from whole blood were analyzed using human HPRT-1 gene-specific primer set. The accuracy of real-time PCR is demonstrated by R² value=0.99482 (Premier qPCR Mix), R² value=0.98909 (iTaq Universal SYBR green supermix) and R² value=0.99233 (TOPreal qPCR 2x premix). Reactions were run on a real-time PCR instrument, Rotor-GENE Q (QIAGEN, Germany).

Figure 3. Comparison of Real-time PCR using Premier 2X qPCR Mix [Green] (= RealHelix™ Premier qPCR Mix [Green]) iTaq Universal SYBR green supermix (company B) and TOPreal qPCR 2x premix (company E). 10-fold serial dilutions of the human genomic DNA (red: 50 ng/rxn, green: 5 ng/rxn, blue: 0.5 ng/rxn) from whole blood were analyzed using human HPRT-1 gene-specific primer set. The accuracy of real-time PCR is demonstrated by R² value=0.999 (RealHelix™ Premier qPCR Mix [Green]), R² value=0.999 (iTaq Universal SYBR green supermix) and R² value=1.000 (TOPreal qPCR 2x premix). Melting curve analysis revealed the high specificity of Premier qPCR Mix. Reactions were run on a real-time PCR instrument, CFX-96 (Bio-Rad, USA). NTC: Negative control.

Figure 4. Amplification of target DNA with various G+C contents using Premier 2X qPCR Mix [Green] (= RealHelix™ Premier qPCR Mix [Green]). Premier 2X qPCR Mix [Green] was evaluated by real-time PCR using 10-fold serial diluted human genomic DNA (red: 50 ng/rxn, green: 5 ng/rxn, blue: 0.5 ng/rxn) with Rotor-Gene Q instrument (QIAGEN, Germany), CFX-96 instrument (Bio-Rad, USA) and ABI7500 real-time PCR system (Applied Biosystems, USA). NTC: Negative control.