Figure 1. Multiple amplification of Five targets in a reaction by Superplex qPCR Premix [Probe] (= RealHelix™ Superplex qPCR Kit [Probe]). 10-fold serial dilutions of human genomic DNA (10, 1, 0.1, and 0.01 ng) were analyzed using human GloB(FAM), AAT(α1 antitypsin, HEX), GAPDH(Cy5), GloB(Cy5.5) specific primer and probe sets. And 10-fold serial dilutions of a plasmid DNA were also added to the reaction mixture and analyzed using a gene-specific primer and probe labeled with Texas Red in the reactions. Reactions were done using the Superplex qPCR Premix [Probe] on a real-time PCR instrument, Bio-Rad CFX96. 

Figure 2. Twelve-targets multiplex amplification in a reaction by Superplex qPCR Premix [Probe] (= RealHelix™ Superplex qPCR Kit [Probe]). Using the allele-specific FMMA probes (labeled with FAM, HEX, Texas Red, or Cy5), twelve rice SNP genotypes were identified by a single reaction. A mix of rice genomic DNAs (total 10ng) was used for the FMMA reaction to detect all of the 12 SNPs screened for the determination of rice variants. The multiplex reaction was done by the Superplex qPCR Premix [Probe] on a real-time PCR instrument, Bio-Rad CFX96.