Figure 1. Multiplex (four targets) qRT-PCR with qRT-PCR Kit [Probe] (= RealHelix™ qRT-PCR Kit [Probe]). Total RNAs from human whole blood were analyzed using four different sets of target-specific primers and probes. The probes were labeled with FAM, HEX, Texas Red, or Cy5. The multiplex reaction was done using the qRT-PCR Kit [Probe] on a real-time PCR instrument, Bio-Rad CFX96. 

Figure 2. Comparison of qRT-PCR Kit [Probe] (= RealHelix™ qRT-PCR Kit [Probe]) and qRT-PCR Kit [Probe type] (the previous version). 10-fold serial dilutions (10, 1, 0.1 and 0.01 ng) of the human total RNA from whole blood were analyzed using human Globin gene specific primer set. qRT-PCR Kit [Probe] shows earlier Ct and more reliable amplifications than the previous version. Reactions were run on a real-time PCR instrument, CFX-96 (Bio-Rad, USA).