Cat.No. | Product | Feature | Size |
---|---|---|---|
BORTA | One-Step RT-PCR Kit [Ab+Taq] | - | Custom |
BORTAU | One-Step RT-PCR Kit [Ab+Taq] , w/UDG | + UDG SYSTEM* | Custom |
One-Step RT-PCR Kit [Ab+Taq] is designed for specific and sensitive amplification of target genes in one-tube reaction from RNA templates. Thermo Reverse Transcriptase, Ab+Taq polymerase, and RNase Inhibitor are supplied as an enzyme mixture. An optimized buffer is provided as a 2x Reaction mix containing a buffer, MgCl2, and dNTPs as 2x concentration. Applied UDG system includes the Heal-labile UDG and dUTP.
NanoHelix的Taq聚合酶是一种高度纯化的重组标准PCR酶。宿主DNA几乎全部从酶中去除,以最大程度减少分子诊断应用中的假阳性反应。一起提供的10x反应缓冲液包含pH缓冲剂,盐,镁和dNTP。
应用
常规PCR和RT-PCR
常规和实时PCR
PCR用于分子诊断
扩增混合物的制造