Cat.No. | Product | Size |
---|---|---|
PP250 | HelixAmp™ Premium-Pfu Polymerase | 250 units |
PP500 | HelixAmp™ Premium-Pfu Polymerase | 500 units |
PP2500 | HelixAmp™ Premium-Pfu Polymerase | 2,500 units |
HelixAmp™ Premium-Pfu polymerase is an editorial enzyme improved from HelixAmp™ Speed-Pfu polymerase. HelixAmp™ Premium-Pfu polymerase shows faster (4 ~ 6 times) polymerization reaction and higher fidelity (3 ~ 5 times) than any other high-fidelity enzymes. With this high speed and accuracy, NanoHelix’s “PMT(polymerase modulator on temperature) technology” is applied in the buffer system, which is effective to reduce primer-dimer formation and non-specific amplification during the PCR. HelixAmp™ Premium-Pfu polymerase is most suitable to faithful amplification of relatively long-ranged target for cloning etc. Due to its high speed, fast PCR with this enzyme could be completed in 30 min for the reliable amplification of less than 1 kb size target DNA. For the maximum performance of PCR reactions high-quality dNTP mixture is supplied. In case of PCR amplification of target DNA with high G+C content or structural problem, such as repeat sequence, the application of N-Solution™ improves the specificity and productivity of the reactions.
NanoHelix的Taq聚合酶是一种高度纯化的重组标准PCR酶。宿主DNA几乎全部从酶中去除,以最大程度减少分子诊断应用中的假阳性反应。一起提供的10x反应缓冲液包含pH缓冲剂,盐,镁和dNTP。
应用
常规PCR和RT-PCR
常规和实时PCR
PCR用于分子诊断
扩增混合物的制造