Figure 1. The express speed of HelixAmp™ Speed-Pfu polymerase. Four different sizes of human gene fragments were amplified with various extension times. PCR reactions were carried out at 95℃, 10 sec and 68℃ for the represented extension time (30 cycles). This result indicates that only 20-23 sec is required for successful synthesis of 1kb size DNA. The total PCR time required for amplification of 1 kb fragment is less than 25 minutes.

Figure 2. A fast PCR with HelixAmp™ Speed-Pfu polymerase and other enzymes. 1 kb fragment of human DNA was amplified from various concentrations of template in a limited extension time, 20 sec. PCR reaction was carried out at 95℃, 10 sec and 68℃, 20 sec (35 cycles).

Figure 3. HelixAmp™ Speed-Pfu polymerase is the enzyme with the highest fidelity. Error Rate = mutation/bp/duplication