Cat.No. | Product | Size |
---|---|---|
PT250 | HelixAmp™ Premium-Taq Polymerase | 250 rxns |
PT500 | HelixAmp™ Premium-Taq Polymerase | 500 rxns |
PT2500 | HelixAmp™ Premium-Taq Polymerase | 2,500 rxns |
HelixAmp™ Premium-Taq polymerase is an anti-Taq antibody complex form of Taq polymerase and ideal for automatic hot-start PCR.The inhibitory anti-Taq antibody inactivates the polymerase by binding at low temperature and suppresses the polymerization from non-specifically bound primers which occurs during the setting of PCR mix and the temperature going up in the PCR machine. At the high temperature of the initial denaturation step of PCR, the antibody is released by denaturation and the free Taq DNA polymerase becomes active. Unlike chemically modified hot-start Taq polymerase, this enzyme needs not prolonged (10 to 15 min.) incubation at above 94℃ to be active. NanoHelix" "PMT(Polymerase n modulator on Temperature) technology" is applied in the buffer system, which is effective to reduce primer-dimer formation and non-specific amplification during the PCR. In case of PCR amplification of target DNA with high G+C content or structural problem, such as repeat sequence, N-Solution™ will improve the specificity and productivity of the reactions.
NanoHelix的Taq聚合酶是一种高度纯化的重组标准PCR酶。宿主DNA几乎全部从酶中去除,以最大程度减少分子诊断应用中的假阳性反应。一起提供的10x反应缓冲液包含pH缓冲剂,盐,镁和dNTP。
应用
常规PCR和RT-PCR
常规和实时PCR
PCR用于分子诊断
扩增混合物的制造