HelixAmp™ Taq-Plus polymerase is an improved form of HelixAmp™ Taq polymerase and amplifies target DNA at broad-range of annealing temperature. NanoHelix’s “PMT (polymerase modulator on temperature) technology” is applied in the buffer system, which is effective to reduce primer-dimer formation and non-specific amplification during the PCR. This enzyme is an optimized blend of HelixAmp™ Taq polymerase and HelixAmp™ Power-Pfu polymerase.HelixAmp™ Taq-Plus polymerase possesses the greater yield, processivity and fidelity than normal Taq polymerase. The fidelity of HelixAmp™ Taq-Plus polymerase is higher approximately 4 times than that of Taq polymerase. In case of PCR amplification of target DNA with high G+C content or structural problem, such as repeat sequence, the application of TuneUp™ solution improves the specificity and productivity of the reaction. 

Figure 1. Comparison of HelixAmp™ Taq-Plus polymerase with other brand of Taq DNA polymerases. 12 different primer sets designed from human genome were used in this PCR. PCR reactions were performed using 10 ng of human genomic DNA under the various annealing temperature. A : NanoHelix, B : Company S[Korea], C : Company T[Japan], D : Company I[Korea].

Figure 2. Limit of detection(LOD) comparison of HelixAmpTM Taq-Plus polymerase with other company’s related products. The indicated primer(POLD2) designed from human genome were used in this PCR. PCR was performed using various concentration of template. A: Company T(Japan), B: Company I(USA), C: NanoHelix(Taq polymerase, D: NanoHelix(Taq-Plus polymerase).

Figure 3. Comparison of HelixAmp™ Taq-Plus polymerase with other company’s related products. Different primer sets(ApoE, MD, Prion, SBMA) designed from the indicated region of human genome were used in this PCR. PCR was performed using 10 ng of human genomic DNA. A: Company T(Ex-Taq), B: Company T(Hot-start Taq), C: Company I(I-Taq), D: Company I(I-Star Taq), E: Company B(Top-Taq), F: Company B(Hot-start Taq), G: Company I(Platinum Taq), H: NnoHelix(Taq), I : NanoHelix(Taq-Plus polymerase).

Contents 

• Taq-Plus polymerase (1.25 units/ul)
• 5x Reaction Mix [PT]
• 5x TuneUp™ solution
• 6x Loading Dye 

Quality control 

• Contamination Assay
• HelixAmp™ Taq-Plus polymerase was passed from quality control assay for contamination of endo- or exodeoxyribonuclease and the bacterial host DNA host.

Storage buffer 

• 50% Glycerol, 20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20
• 0.5% Nonidet P-40, 1 mM PMSF