Cat.No. | Product | Size |
---|---|---|
DFT250 | HelixAmp™ DNA-free Taq Polymerase | 250 units |
DFT500 | HelixAmp™ DNA-free Taq Polymerase | 500 units |
DFT2500 | HelixAmp™ DNA-free Taq Polymerase | 2,500 units |
DFT250N | HelixAmp™ DNA-free Taq Polymerase (with dNTP Mix) | 250 units |
DFT500N | HelixAmp™ DNA-free Taq Polymerase (with dNTP Mix) | 500 units |
DFT2500N | HelixAmp™ DNA-free Taq Polymerase (with dNTP Mix) | 2,500 units |
DFHT125 | HelixAmp™ DNA-free Hot-Taq Polymerase | 125 units |
DFHT250 | HelixAmp™ DNA-free Hot-Taq Polymerase | 250 units |
DFHT125N | HelixAmp™ DNA-free Hot-Taq Polymerase (with dNTP Mix) | 125 units |
DFHT 250N | HelixAmp™ DNA-free Hot-Taq Polymerase (with dNTP Mix) | 250 units |
HelixAmp™ DNA-free Taq polymerase is a recombinant enzyme expressed and purified from a bacterial host cell harboring Thermus aquaticus DNA polymerase gene and purified using a process that minimizes level of contaminating host DNA. HelixAmp™ DNA-free Taq polymerase is high quality DNA polymerase and suitable for molecular diagnostics. Because the contaminating host genomic DNA can cause the false positive results, use of DNA-free Taq polymerase is very important in PCR detection of bacteria with universal primers(e.g. 16s rRNA specific primers). A chemically modified hot-start version of this enzyme, HelixAmp™ DNA-free Hot-Taq polymerase, is also available. (see the product list)
NanoHelix的Taq聚合酶是一种高度纯化的重组标准PCR酶。宿主DNA几乎全部从酶中去除,以最大程度减少分子诊断应用中的假阳性反应。一起提供的10x反应缓冲液包含pH缓冲剂,盐,镁和dNTP。
应用
常规PCR和RT-PCR
常规和实时PCR
PCR用于分子诊断
扩增混合物的制造