Cat.No. | Product | Size |
---|---|---|
RT10K | HelixCript™ Thermo Reverse Transcriptase | 10,000 units |
RT50K | HelixCript™ Thermo Reverse Transcriptase | 50,000 units |
RT10KN | HelixCript™ Thermo Reverse Transcriptase (with dNTP Mix) | 10,000 units |
RT50KN | HelixCript™ Thermo Reverse Transcriptase (with dNTP Mix) | 50,000 units |
HelixCript™ Thermo Reverse Transcriptase, a Thermo stable and RNase H negative variant of M-MLV RTase, can synthesize cDNAs from purified RNA templates at a temperature range of 42℃ ~ 55℃ and especially shows the highest activity at 50℃. At the escalated temperature, the cDNA synthesis is enhanced partially due to the less internal structural formation of the template RNA and increased polymerization activity of RTase. The high processivity and productivity of HelixCript™ Thermo Reverse Transcriptase allows this enzyme to amplify the high yield of product and can synthesize cDNA of target gene up to 12 kb from RNA template. This enzyme is quite suitable to synthesize the first-strand cDNA for RT-PCR of target gene. The cDNA synthesis from total RNA or poly-(A)+ RNA is performed by random primer, oligo-d(T) primer, or gene-specific primer.
NanoHelix的Taq聚合酶是一种高度纯化的重组标准PCR酶。宿主DNA几乎全部从酶中去除,以最大程度减少分子诊断应用中的假阳性反应。一起提供的10x反应缓冲液包含pH缓冲剂,盐,镁和dNTP。
应用
常规PCR和RT-PCR
常规和实时PCR
PCR用于分子诊断
扩增混合物的制造