Cat.No. | Product | Size |
---|---|---|
DPR200 | HelixAmp™ Direct PCR [3G] | 200 rxns |
DPRU200 | HelixAmp™ Direct PCR [3G] [Containing UDG/dUTP] | 200 rxns |
DPRH200 | HelixAmp™ Direct PCR [3G] (Hot-start) | 200 rxns |
DPRHU200 | HelixAmp™ Direct PCR [3G] (Hot-start) [Containing UDG/dUTP] | 200 rxns |
HelixAmp™ Direct PCR [3G] is designed for the PCR amplification directly from animal tissues, whole blood, and plant tissues without any DNA purification processes. This kit contains a 2x reaction mix including Taq DNA polymerase, dNTPs, MgCl2, and unique buffer system to resist various PCR inhibitors of tissue samples. HelixAmp™ Direct PCR [3G] (Hot-start) is including hot-start Taq DNA polymerase that is inactive at lower temperature by an inhibitory antibody and using this enzyme avoids the polymerization from non-specifically bound primers during the setting of PCR mix and at the start of PCR cycles. At high temperature of the initial denaturation step of PCR, the inhibitory antibody is released by denaturation and the free Taq DNA polymerase becomes active. NanoHelix’s Direct PCR [3G] is based on Taq polymerase. Due to this enzyme’s robust amplification and 3’ to 5’ exo-negative properties, this kit could be used for allele specific PCR which is routinely used for various genotyping. Moreover the Uracil-DNA glycosylase with dUTP system for the prevention of carryover contamination can be applied to this Taq polymerase-based kit. dUTP could not be used with the Pfu DNA polymerase and its derivatives. A Uracil-DNA glycosylase and dUTP applied version is also available.
NanoHelix的Taq聚合酶是一种高度纯化的重组标准PCR酶。宿主DNA几乎全部从酶中去除,以最大程度减少分子诊断应用中的假阳性反应。一起提供的10x反应缓冲液包含pH缓冲剂,盐,镁和dNTP。
应用
常规PCR和RT-PCR
常规和实时PCR
PCR用于分子诊断
扩增混合物的制造