Hot-Taq polymerase is a chemically-modified form of purified Taq DNA polymerase and quite suitable for high-specific hot-start PCR, real-time PCR and multiplex PCR. The 10x reaction buffer supplied together contains pH-buffering agent, salts, magnesium, and dNTPs.
Application
- Hot-start PCR
- Multiplex PCR
- Real-time PCR
- High specific and sensitive PCR
- Amplification of complex DNA and cDNA
- PCR for molecular diagnostics
Enzyme activities: Highly processive 5"-3" DNA polymerase; double-strand specific 5"-3" exonuclease; no 3"-5" exonuclease activity
Enzyme activation: 15 min at +95°C
Storage buffer: 20 mM Tris-HCl (pH 9.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween-20 and 50% (v/v) glycerol
DNase contamination test: Not detectable (Incubation with 40U enzyme and pUC19 plasmid at 37°C, 1hr)
RNase contamination test: Not detectable (Incubation with 40U enzyme and Human total RNA at 37°C, 1hr)
Activity test in PCR and qPCR: Correspond to reference (Human genomic DNA target)
Stability: 24 months at -20°C