Figure 1. Isolation of total RNA from animal or plant tissue samples using PureHelix™ Total RNA Purification Kit.Total RNAs were isolated from 30 mg of rat liver tissue, or 20 mg of tobacco leaf according to the manual. The extractions were duplicated and 1 μl from each 50 μl eluted solutions were analyzed on 1% agarose gel electrophoresis.

Figure 2. Real-time PCR analysis of Hepatitis A virus(HAV) for total RNAs purified from HAV-positive serum using PureHelix™ Total RNA Purification Kit. 10-fold serial-diluted total RNA purified from HAV-positive serum were analyzed using the RealHelix™ qRT-PCR Kit.

Figure 3. Viral RNA detection from total RNA isolated by PureHelix™ Total RNA Purification Kit. Total RNA was isolated from a nasopharyngeal specimen of new influenza virus-infected patient and one-step RT-PCR was performed for the target corresponding to each influenza A (InfA, 106 bp), Swine Influenza A(SW InfA, 195 bp), Swine Influenza H1(SW H1, 116 bp), or RNase P (internal control, 65 bp).
Application
- Preparation of total RNA for RT-PCR or quantitative RT-PCR
- Preparation of total RNA sample for molecular diagnostics
Quality Control
PureHelix™ Total RNA Purification Kit [Virus, Animal, Plant, Bacteria] was tested for the isolation of total RNA from blood, animal tissue, plant leaf tissue and bacterial cell.