Thermo Reverse Transcriptase, a thermostable and RNase H-negative variant of M-MLV Reverse Transcriptase (RTase), is an enzyme to synthesize cDNAs from RNA templates at a temperature range of 42℃ ~ 55℃ and shows the highest activity at 50℃. The high processivity and productivity of Thermo Reverse Transcriptase allows to amplify the high yield of product and can synthesize cDNA of target gene up to 12 kb from an RNA template.  

Application 

• Generation of the first-strand cDNA up to 12 kb for libraries or cloning
• Two-step or One-step RT-PCR applications 
• Conventional or real-time RT-PCR 
• RT-PCR for detection of viral RNA

Enzyme activities: RNA-dependent DNA polymerase. No RNase H activity 

Recommended reaction temperature: 42 to 65°C 

Storage buffer: 20mM Tris-HCl (pH7.5), 0.1mM EDTA-NaOH (pH 8.0), 100mM KCl, 0.01% Nonidet P-40, 1mM DTT, 50% Glycerol

Purity: ≥90% (SDS-PAGE)

DNase contamination test: Not detectable (Incubation with 200U enzyme and pUC19 plasmid at 37°C, 1hr) 

RNase contamination test: Not detectable (Incubation with 200U enzyme and Human total RNA at 37°C, 1hr)

Activity test in cDNA synthesis, RT-PCR and qRT-PCR: Correspond to reference (Human RNA target)

Stability: 24 months at -20°C.